Sun Jia-Lei, Zhu Bao-Song, Gong Wei, Zhang Peng, Yu Li-Yan, Zhao Kui, Xing Chun-Gen
Department of General Surgery, The Second Affiliated Hospital, Soochow University, Suzhou 215004, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2011 May;14(5):364-7.
To investigate the effect of phosphatidylinositol 3-kinase inhibitor LY294002 combined with NF-κB P65 nuclear translocation inhibitor SN50 on the tumor cell growth and apoptosis using a nude mouse model of gastric cancer.
Human gastric cancer cell strain SGC7901 was transplanted subcutaneously to nude mice to establish tumor models. Model mice were randomly divided into the control group, the LY294002 treatment group, the SN50 treatment group, and the LY294002+SN50 treatment group, with 5 in each group. After being treated for 10 days, the inhibition rate of tumor growth was ascertained by measuring the size of tumor. Immunohistochemical method was used to detect the expression levels of Bcl-2, P53 and Bax proteins and transmission electron microscopy to investigate the apoptosis of tumor cells.
On the 10th day after treatment, the inhibition rate of gastric cancer cellular growth in the LY294002+SN50 group was (49.2±2.5)%, which was significantly higher than that in the LY294002 group(29.4±1.5)% and SN50 group (19.7±1.6)%(P<0.05). In comparison with the other two groups, LY294002+SN50 group exhibited more severe apoptosis, with expression of Bcl-2 decreased and that of P53 and Bax increased more significantly(P<0.05).
LY294002 combined with SN50 inhibits the growth of SGC7901 transplanted tumor and aggravates the apoptosis of gastric cancer cells in nude mice model.
利用胃癌裸鼠模型研究磷脂酰肌醇3激酶抑制剂LY294002联合核因子κB P65核转位抑制剂SN50对肿瘤细胞生长及凋亡的影响。
将人胃癌细胞株SGC7901皮下移植到裸鼠体内建立肿瘤模型。将模型小鼠随机分为对照组、LY294002治疗组、SN50治疗组和LY294002+SN50治疗组，每组5只。治疗10天后，通过测量肿瘤大小确定肿瘤生长抑制率。采用免疫组织化学方法检测Bcl-2、P53和Bax蛋白的表达水平，并通过透射电子显微镜观察肿瘤细胞凋亡情况。
治疗第10天，LY294002+SN50组胃癌细胞生长抑制率为(49.2±2.5)%，显著高于LY294002组(29.4±1.5)%和SN50组(19.7±1.6)%(P<0.05)。与其他两组相比，LY294002+SN50组凋亡更严重，Bcl-2表达降低，P53和Bax表达升高更显著(P<0.05)。
LY294002联合SN50可抑制裸鼠模型中SGC7901移植瘤的生长，并加重胃癌细胞凋亡。
