马铃薯脂氧合酶将二高-γ-亚麻酸转化为单羟基酸和二羟基酸：8,9-白三烯A3形成的证据。

Conversion of dihomo-gamma-linolenic acid to mono- and dihydroxy acids by potato lipoxygenase: evidence for the formation of 8,9-leukotriene A3.

作者信息

Reddy C C, Bertler C, Hammarström S

机构信息

Department of Cell Biology, Faculty of Health Sciences, University of Linköping, Sweden.

出版信息

Arch Biochem Biophys. 1990 Jun;279(2):211-7. doi: 10.1016/0003-9861(90)90483-f.

DOI:10.1016/0003-9861(90)90483-f

PMID:2161640

Abstract

Evidence for the formation of a positional isomer of leukotriene (LT) C3 (8,9-LTC3) from dihomo-gamma-linolenic acid has been published (Hammarström, S. J. Biol. Chem. 256, 7712-7714, 1981). This report describes the conversion of dihomo-gamma-linolenic acid to a postulated intermediate in former reaction, 8,9-LTA3, by purified lipoxygenase from potato tubers. 8(S)-Hydroperoxyeicosatrienoic acid (8(S)-HPETrE) was the most abundant dioxygenation product formed followed by 11-, 15-, and 12-HPETrEs (in decreasing order of abundance). In addition, 8(S),15(S)- plus 8(S), 15(R)-dihydroperoxyeicosatetraenoic acid (DiHPE-TrE) (EZE), and 8(S),15(S)- plus 8(S),15(R)-dihydroxy-eicosatetraenoic acid (DiHETrE) (EEE) were generated. Under anaerobic conditions only the latter two isomers of 8,15-DiHETrE (EEE) were obtained from 8-HPETrE. The results suggest that 8,9-LTA3 is synthesized by the sequential action of 8- and 11-lipoxygenase activities associated with the potato enzyme.

摘要

关于从二高-γ-亚麻酸形成白三烯（LT）C3的位置异构体（8,9-LTC3）的证据已经发表（哈马斯特伦，S. 《生物化学杂志》256，7712 - 7714，1981）。本报告描述了通过从马铃薯块茎中纯化的脂氧合酶将二高-γ-亚麻酸转化为前一反应中假定的中间体8,9-LTA3的过程。8（S）-氢过氧化二十碳三烯酸（8（S）-HPETrE）是形成的最丰富的双加氧产物，其次是11-、15-和12-HPETrEs（按丰度递减顺序）。此外，还生成了8（S），15（S）-加8（S），15（R）-二氢过氧化二十碳四烯酸（DiHPE-TrE）（EZE）和8（S），15（S）-加8（S），15（R）-二羟基二十碳四烯酸（DiHETrE）（EEE）。在厌氧条件下，仅从8-HPETrE获得了后两种8,15-DiHETrE（EEE）异构体。结果表明，8,9-LTA3是由与马铃薯酶相关的8-和11-脂氧合酶活性的顺序作用合成的。