Department of Pathology, Dalian Medical University, Dalian, Dalian 116044, China.
Biomed Pharmacother. 2011 Jun;65(3):157-62. doi: 10.1016/j.biopha.2011.02.010. Epub 2011 May 5.
We have previously demonstrated that enoyl coenzyme A hydratase 1 (Ech1) is involved in the lymphatic metastasis of tumors. In this study, RNAi was used to investigate the role of Ech1 in Hca-F, a hepatocarcinoma cell line with high rates of lymphatic metastasis. The downregulation of Ech1 inhibited proliferation of the Hca-F cells, increased the ratio of Hca-F cells in S phase to G(1) phase and decreased the adhesion and migration capacities of Hca-F cells. A higher expression level of Ech1 was confirmed in tissue from patients with gastric carcinoma (GC) with lymph node metastases (LNM), indicating the clinical association with tumor metastasis. The results indicate that Ech1 is a critical factor in the development of lymphatic metastasis in these tumors.
我们之前已经证明烯酰辅酶 A 水合酶 1（Ech1）参与了肿瘤的淋巴转移。在这项研究中，使用 RNAi 来研究 Ech1 在具有高淋巴转移率的肝癌细胞系 Hca-F 中的作用。Ech1 的下调抑制了 Hca-F 细胞的增殖，增加了 Hca-F 细胞在 S 期到 G1 期的比例，并降低了 Hca-F 细胞的黏附和迁移能力。在有淋巴结转移（LNM）的胃癌（GC）患者的组织中，证实了 Ech1 的表达水平更高，表明其与肿瘤转移具有临床相关性。结果表明，Ech1 是这些肿瘤中淋巴转移发展的关键因素。
