Vellaiswamy Manohari, Campagna Bernard, Raoult Didier
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, URMITE, CNRS UMR 6236-IRD 198, Faculté de Médecine, IFR48, Università de la Méditerranée, Marseille, France.
New Microbiol. 2011 Apr;34(2):209-18. Epub 2011 Apr 30.
Rickettsia conorii, the etiologic agent of Mediterranean spotted fever, belongs to the spotted fever group of Rickettsia. It is an obligate intracellular bacterium that grows within the cytoplasm of its eukaryotic host cells. It is motile in the cytoplasm of infected cells and RickA is reported as critical protein in this aspect. However, the subcellular localization of RickA remains uncertain. We describe a simple method allowing RickA protein to be localized by immunofluorescence assay (IFA) and transmission electron microscopy (TEM). By using IFA we showed the global expression of surface protein RickA in R. conorii organisms. The TEM results showed that RickA is widely expressed over the entire bacterial surface of R. conorii.
康氏立克次体是地中海斑疹热的病原体，属于立克次体属斑疹热群。它是一种专性细胞内细菌，在其真核宿主细胞的细胞质内生长。它在受感染细胞的细胞质中具有运动性，据报道RickA在这方面是关键蛋白。然而，RickA的亚细胞定位仍不确定。我们描述了一种简单的方法，可通过免疫荧光测定法（IFA）和透射电子显微镜（TEM）对RickA蛋白进行定位。通过IFA，我们展示了表面蛋白RickA在康氏立克次体中的整体表达。TEM结果表明，RickA在康氏立克次体的整个细菌表面广泛表达。
