Department of Biology, College of Basic Sciences, Science and Research Branch, Islamic Azad University, Pounak, Tehran, Iran.
Appl Biochem Biotechnol. 2011 Sep;165(2):572-82. doi: 10.1007/s12010-011-9276-1. Epub 2011 May 27.
In this study, we used sugars as stabilizing additives to improve the thermostability and to inhibit aggregation of firefly luciferase. The combination of sucrose and trehalose has a strong stabilizing effect on firefly luciferase activity and prevents its thermoinactivation. These additives can also increase optimum temperature. It has been shown that the presence of both sucrose and trehalose can inhibit thermal aggregation of firefly luciferase and decrease bioluminescence decay rate. In order to understand the molecular mechanism of thermostabilization, we investigated the effects of sucrose and trehalose combination on the secondary structure of luciferase by Fourier transform infrared spectroscopy. Minor changes in content of secondary structure of firefly luciferase are observed upon treatment with additives.
在这项研究中，我们使用糖作为稳定剂来提高萤火虫荧光素酶的热稳定性和抑制其聚集。蔗糖和海藻糖的组合对萤火虫荧光素酶的活性有很强的稳定作用，并防止其热失活。这些添加剂还可以提高最适温度。结果表明，蔗糖和海藻糖的存在可以抑制萤火虫荧光素酶的热聚集并降低生物发光衰减率。为了了解热稳定化的分子机制，我们通过傅里叶变换红外光谱研究了蔗糖和海藻糖组合对荧光素酶二级结构的影响。在添加添加剂后，萤火虫荧光素酶的二级结构含量略有变化。
