Chatterjee A, Bhattacharya A K
Department of Biophysics, Banaras Hindu University, Varanasi, India.
Indian J Exp Biol. 1990 Apr;28(4):397-8.
Cyclic AMP binding in the extract of E. coli was taken as a measure of cAMP receptor protein (CRP) present in the cells. The gamma-irradiation of cells caused a dose-dependent inhibition of cAMP binding activity indicating that CRP gene is affected by gamma-irradiation. The binding activity in cells irradiated with 240 Gy gamma-ray dose remained unaltered by post-irradiation incubation. This supports previous finding that the enhanced inhibition of the L-arabinose isomerase synthesizing capacity, following incubation of gamma-irradiated cells at higher doses, must be due to a different cause than the catabolite repression.
大肠杆菌提取物中的环磷酸腺苷（cAMP）结合被用作细胞中存在的cAMP受体蛋白（CRP）的衡量指标。细胞的γ射线照射导致cAMP结合活性呈剂量依赖性抑制，表明CRP基因受γ射线照射影响。用240 Gyγ射线剂量照射的细胞中的结合活性在照射后孵育时保持不变。这支持了先前的发现，即γ射线照射的细胞在较高剂量下孵育后，L -阿拉伯糖异构酶合成能力的增强抑制一定是由不同于分解代谢物阻遏的原因引起的。
