Department of Surgical and Medical Disciplines Gerontology Section, University of Torino-Italy, Corso Bramante 88/90, 10126 Torino, Italy.
Osteoporos Int. 2012 Apr;23(4):1245-53. doi: 10.1007/s00198-011-1666-2. Epub 2011 May 27.
This study shows that teriparatide promotes the circulating osteoblast (OB) precursor degree of maturation in patients affected by postmenopausal osteoporosis.
Anabolic treatment with teriparatide has proven effective for the therapy of postmenopausal osteoporosis and significantly reduces the risk of non-vertebral fragility fractures. The aim of this study was to investigate the effect of teriparatide on circulating OB precursors.
We evaluated by flow cytometry and real-time PCR the expression of OBs typical markers in peripheral blood mononuclear cells during treatment with teriparatide plus calcium and vitamin D, raloxifene plus calcium and vitamin D or calcium and vitamin D alone at various time points. Serum bone alkaline phosphatase and osteocalcin (OC) were measured as markers of bone turnover.
Our results show that circulating OB precursors are more numerous and more immature in patients affected by fragility fractures than in osteoporotic patients without fractures. We also show that teriparatide treatment increases the expression of alkaline phosphatase and of OC in OB precursors; thus, it increases their degree of maturation.
We suggest that teriparatide acts as anabolic agents also by promoting the maturation of OB precursors.
本研究表明，特立帕肽可促进绝经后骨质疏松症患者循环成骨细胞（OB）前体的成熟度。
特立帕肽的合成代谢治疗已被证明对绝经后骨质疏松症的治疗有效，可显著降低非椎体脆性骨折的风险。本研究旨在探讨特立帕肽对循环 OB 前体的影响。
我们通过流式细胞术和实时 PCR 评估了特立帕肽联合钙和维生素 D、雷洛昔芬联合钙和维生素 D 或单独钙和维生素 D 治疗时，外周血单个核细胞中 OB 典型标志物的表达，同时检测了骨碱性磷酸酶和骨钙素（OC）等骨转换标志物的血清水平。
我们的结果表明，脆性骨折患者的循环 OB 前体数量更多，且不成熟程度更高，而非骨折骨质疏松症患者的循环 OB 前体数量较少，成熟程度更高。我们还表明，特立帕肽治疗可增加 OB 前体中碱性磷酸酶和 OC 的表达，从而增加其成熟度。
我们认为，特立帕肽还通过促进 OB 前体的成熟来发挥其合成代谢作用。
