Berthod A, Mallet A I, Bully M
Laboratoire des Sciences Analytiques, Université Claude Bernard, Lyon 1, UA CNRS 435 (J. M. Mermet), 69622 Villeurbanne cedex, France.
Anal Chem. 1996 Feb 1;68(3):431-6. doi: 10.1021/ac950409k.
Countercurrent chromatography (CCC) is a chromatographic separation technique that uses a liquid as a stationary phase. Centrifugal forces are used to immobilize the liquid stationary phase when the liquid mobile phase is pushed through it. In CCC, the solutes are separated according to their liquid-liquid partition coefficients. The solutes studied were the alkylbenzene homologues from benzene to hexylbenzene and some polyaromatic hydrocarbons (PAHs) from naphthalene to coronene. Their liquid-liquid partition coefficients were measured in the five waterless biphasic systems formed by heptane, as the apolar liquid phase of the five biphasic systems, and four dipolar aprotic solvents, dimethyl sulfoxide, dimethylformamide, furfural, and N-methylpyrrolidone, and the polar proton-donor solvent methanol. The coefficients were compared to the corresponding capacity factors obtained by classical liquid chromatography on octadecyl-bonded silica. For the five biphasic solvent systems studied, linear relationships were found between the partition coefficients and the sp(3) and sp(2) hybridized carbon atom number for the alkylbenzene and PAH series, respectively. The sp(2) and sp(3) transfer energies were estimated, and their ratio was used to quantify the solvent selectivity toward aromatic extraction.
逆流色谱法（CCC）是一种色谱分离技术，它使用液体作为固定相。当液体流动相被推动通过时，利用离心力固定液体固定相。在CCC中，溶质根据其液 - 液分配系数进行分离。所研究的溶质是从苯到己基苯的烷基苯同系物以及从萘到晕苯的一些多环芳烃（PAH）。它们的液 - 液分配系数是在由庚烷（作为五个双相系统的非极性液相）与四种偶极非质子溶剂（二甲基亚砜、二甲基甲酰胺、糠醛和N - 甲基吡咯烷酮）以及极性质子供体溶剂甲醇形成的五个无水双相系统中测量的。将这些系数与通过在十八烷基键合硅胶上进行经典液相色谱法获得的相应容量因子进行比较。对于所研究的五个双相溶剂系统，分别在烷基苯和PAH系列的分配系数与sp(3)和sp(2)杂化碳原子数之间发现了线性关系。估算了sp(2)和sp(3)转移能量，并使用它们的比率来量化溶剂对芳烃萃取的选择性。
