Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
Biochem Biophys Res Commun. 2011 Jun 17;409(4):634-9. doi: 10.1016/j.bbrc.2011.05.055. Epub 2011 May 17.
The synthesis of JA-Ile was catalysed by JA-Ile synthase, which is a member of the group I GH3 family of proteins. Here, we showed evidence that OsGH3.5 (OsJAR1) and OsGH3.3 (OsJAR2) are the functional JA-Ile synthases in rice, using recombinant proteins. The expression levels of OsJAR1 and OsJAR2 were induced in response to wounding with the concomitant accumulation of JA-Ile. In contrast, only the expression of OsJAR1 was associated with the accumulation of JA-Ile after blast infection. Our data suggest that these two JA-Ile synthases are differentially involved in the activation of JA signalling in response to wounding and pathogen challenge in rice.
JA-Ile 的合成由 JA-Ile 合酶催化，后者是 GH3 家族 I 蛋白的成员。在这里，我们使用重组蛋白证明了 OsGH3.5（OsJAR1）和 OsGH3.3（OsJAR2）是水稻中具有功能的 JA-Ile 合酶。OsJAR1 和 OsJAR2 的表达水平在受到创伤后被诱导，同时 JA-Ile 积累。相比之下，只有 OsJAR1 的表达与爆炸感染后 JA-Ile 的积累相关。我们的数据表明，这两种 JA-Ile 合酶在水稻对创伤和病原体挑战的 JA 信号激活中存在差异。
