Fukushima Y, Yanagisawa M, Nakamoto Y, Miura A B
Third Department of Internal Medicine, Akita University School of Medicine.
Tohoku J Exp Med. 1990 Feb;160(2):129-40. doi: 10.1620/tjem.160.129.
Erythropoietin (EPO) producing cells were examined by the immunofluorescent antibody technique, and the alterations in the Ia antigen expression in glomeruli by hypoxic stimuli. Antisera were obtained from rabbits immunized with pure human EPO. Positive fluorescence was localized in glomerular mesangial regions of the hypoxic rat kidney. Although hypoxia showed neither increase in the number of glomerular cells nor hypertrophy of the mesangial matrix, it induced an increase of the Ia antigen expressions in glomeruli. The peak of the increase occurred 2 hr after hypoxia and then decreased until 24 hr later. The increase of Ia antigen after 2 hr of hypoxic stimuli was 176.5 +/- 7.0% of normal controls, and ranged from 151 to 209%. In conclusion, Ia positive cells in glomeruli may have some relationship to the stimulation of EPO production.
采用免疫荧光抗体技术检测促红细胞生成素（EPO）产生细胞，并观察低氧刺激下肾小球Ia抗原表达的变化。抗血清取自用纯人EPO免疫的兔。在低氧大鼠肾脏的肾小球系膜区发现阳性荧光。尽管低氧既未使肾小球细胞数量增加，也未使系膜基质肥大，但却诱导了肾小球Ia抗原表达增加。这种增加在低氧后2小时达到峰值，随后直至24小时后下降。低氧刺激2小时后Ia抗原增加量为正常对照的176.5±7.0%，范围在151%至209%之间。总之，肾小球中Ia阳性细胞可能与EPO产生的刺激存在某种关系。
