Rwambo P M, Issel C J, Adams W V, Hussain K A, Miller M, Montelaro R C
Department of Veterinary Microbiology and Parasitology, Louisiana State University, School of Veterinary Medicine, Baton Rouge.
Arch Virol. 1990;111(3-4):199-212. doi: 10.1007/BF01311054.
Three ponies were inoculated with plasma containing 10(4.8) TCID50 of equine infectious anemia virus (EIAV) and observed for 165 to 440 days. Each pony developed a febrile response within 3 weeks of infection during which a plasma viremia greater than or equal to 10(3.5) TCID50/ml was observed. Analyses of four isolates from sequential febrile episodes in a single pony were conducted by two-dimensional tryptic peptide maps and with monoclonal antibodies in immunoblots. Structural and antigenic alterations were observed in the envelope glycoproteins gp90 and gp45, with greatest variation in gp90. Specific IgG to EIAV gp90, gp45, and p26 of homologous and heterologous isolates was detectable by immunoblots within one month after infection although IgG levels to gp45 at this time were relatively low. The group-specific determinants of gp90 and gp45 were more antigenic than those of p26; however, binding of IgG to these determinants did not correlate with neutralization of EIAV as assayed in fetal equine kidney cells. Neutralizing antibodies were first detectable within two months of infection and only neutralized viruses isolated prior to serum collection. Neutralizing activity of sera collected later in the infection was broadly reactive regardless of the number of clinical episodes the donor had suffered.
三只小马接种了含有10(4.8) 半数组织培养感染剂量（TCID50）马传染性贫血病毒（EIAV）的血浆，并观察了165至440天。每只小马在感染后3周内出现发热反应，在此期间观察到血浆病毒血症大于或等于10(3.5) TCID50/ml。通过二维胰蛋白酶肽图谱和免疫印迹中的单克隆抗体，对一匹小马连续发热发作的四个分离株进行了分析。在包膜糖蛋白gp90和gp45中观察到结构和抗原性改变，其中gp90变化最大。感染后一个月内，通过免疫印迹可检测到针对同源和异源分离株的EIAV gp90、gp45和p26的特异性IgG，尽管此时针对gp45的IgG水平相对较低。gp90和gp45的群特异性决定簇比p26的更具抗原性；然而，IgG与这些决定簇的结合与在马胎儿肾细胞中测定的EIAV中和作用不相关。中和抗体在感染后两个月内首次可检测到，并且仅中和血清采集前分离的病毒。感染后期采集的血清的中和活性具有广泛的反应性，无论供体经历的临床发作次数如何。
