Dept. of Biomedical Sciences, E102 Veterinary Medicine, 1600 E. Rollins Rd., Univ. of Missouri, Columbia, MO 65211, USA.
Am J Physiol Heart Circ Physiol. 2011 Aug;301(2):H555-64. doi: 10.1152/ajpheart.00065.2011. Epub 2011 May 27.
While the salutary effects of exercise training on conduit artery endothelial cells have been reported in animals and humans with cardiovascular risk factors or disease, whether a healthy endothelium is alterable with exercise training is less certain. The purpose of this study was to evaluate the impact of exercise training on transcriptional profiles in normal endothelial cells using a genome-wide microarray analysis. Brachial and internal mammary endothelial gene expression was compared between a group of healthy pigs that exercise trained for 16-20 wk (n = 8) and a group that remained sedentary (n = 8). We found that a total of 130 genes were upregulated and 84 genes downregulated in brachial artery endothelial cells with exercise training (>1.5-fold and false discovery rate <15%). In contrast, a total of 113 genes were upregulated and 31 genes downregulated in internal mammary artery endothelial cells using the same criteria. Although there was an overlap of 66 genes (59 upregulated and 7 downregulated with exercise training) between the brachial and internal mammary arteries, the identified endothelial gene networks and biological processes influenced by exercise training were distinctly different between the brachial and internal mammary arteries. These data indicate that a healthy endothelium is indeed responsive to exercise training and support the concept that the influence of physical activity on endothelial gene expression is not homogenously distributed throughout the vasculature.
虽然运动训练对心血管危险因素或疾病患者的大血管内皮细胞有益作用已在动物和人群中得到证实，但健康内皮细胞是否可以通过运动训练来改变尚不确定。本研究旨在通过全基因组微阵列分析评估运动训练对正常内皮细胞转录谱的影响。对一组经过 16-20 周运动训练的健康猪（n=8）和一组久坐不动的猪（n=8）的肱动脉和内乳动脉内皮基因表达进行了比较。我们发现，运动训练使肱动脉内皮细胞中共有 130 个基因上调和 84 个基因下调（>1.5 倍，假发现率<15%）。相比之下，使用相同标准，内乳动脉内皮细胞中有 113 个基因上调和 31 个基因下调。尽管肱动脉和内乳动脉之间有 66 个基因重叠（运动训练使 59 个基因上调，7 个基因下调），但运动训练影响的内皮基因网络和生物学过程在肱动脉和内乳动脉之间存在明显差异。这些数据表明，健康的内皮细胞确实对运动训练有反应，并支持这样一种观点，即体力活动对内皮基因表达的影响并非均匀分布于整个血管系统。
