Okazawa A, Kobayashi H, Adachi M, Takahashi T, Misawa M
First Department of Internal Medicine, School of Medicine, Showa University, Tokyo, Japan.
Nihon Kyobu Shikkan Gakkai Zasshi. 1990 Feb;28(2):293-9.
To clarify the mechanisms of ozone-induced airway hyperresponsiveness, we studied the effect of leukotriene C4/D4 receptor antagonist (ONO-1078) and thromboxane A2 synthetase inhibitor (OKY-046) on airway hyperresponsiveness induced by ozone exposure in guinea pigs. Airway responsiveness to inhaled methacholine was determined in artificially ventilated guinea pigs using a modification of the Konzett-Rössler technique. After the methacholine challenge, bronchoalveolar lavage (BAL) was performed. After 1 hour following ozone exposure (2.9 ppm, 30 min), airway responsiveness increased significantly. There was no cellular change in the bronchoalveolar lavage fluid (BALF). Pretreatment with ONO-1078 (30 mg/kg, i.p.) or OKY-046 (20 mg/kg, i.p.) caused no effect on airway responsiveness before ozone exposure, and inhibited the increase of airway responsiveness induced by ozone exposure. These results suggest that leukotriene and thromboxane play an important role in the development of airway hyperresponsiveness induced by ozone exposure in guinea pigs.
为阐明臭氧诱导气道高反应性的机制，我们研究了白三烯C4/D4受体拮抗剂（ONO-1078）和血栓素A2合成酶抑制剂（OKY-046）对豚鼠臭氧暴露诱导的气道高反应性的影响。使用改良的Konzett-Rössler技术，在人工通气的豚鼠中测定气道对吸入乙酰甲胆碱的反应性。乙酰甲胆碱激发后，进行支气管肺泡灌洗（BAL）。臭氧暴露（2.9 ppm，30分钟）1小时后，气道反应性显著增加。支气管肺泡灌洗液（BALF）中无细胞变化。用ONO-1078（30 mg/kg，腹腔注射）或OKY-046（20 mg/kg，腹腔注射）预处理在臭氧暴露前对气道反应性无影响，并抑制了臭氧暴露诱导的气道反应性增加。这些结果表明，白三烯和血栓素在豚鼠臭氧暴露诱导的气道高反应性发展中起重要作用。
