Filippova Ekaterina V, Chruszcz Maksymilian, Cymborowski Marcin, Gu Jun, Savchenko Alexei, Edwards Aled, Minor Wladek
Department of Molecular Physiology and Biological Physics, University of Virginia, 1340 Jefferson Park Avenue, Jordan Hall, Room 4223, Charlottesville, VA 22908, USA.
J Struct Funct Genomics. 2011 Sep;12(3):149-57. doi: 10.1007/s10969-011-9112-4. Epub 2011 May 29.
A structure of the apo-form of the putative transcriptional regulator SCO0520 from Streptomyces coelicolor A3(2) was determined at 1.8 Å resolution. SCO0520 belongs to the TetR family of regulators. In the crystal lattice, the asymmetric unit contains two monomers that form an Ω-shaped dimer. The distance between the two DNA-recognition domains is much longer than the corresponding distances in the known structures of other TetR family proteins. In addition, the subunits in the dimer have different conformational states, resulting in different relative positions of the DNA-binding and regulatory domains. Similar conformational modifications are observed in other TetR regulators and result from ligand binding. These studies provide information about the flexibility of SCO0520 molecule and its putative biological function.
在1.8埃分辨率下测定了来自天蓝色链霉菌A3(2)的假定转录调节因子SCO0520的脱辅基形式的结构。SCO0520属于TetR家族调节因子。在晶格中，不对称单元包含两个形成Ω形二聚体的单体。两个DNA识别结构域之间的距离比其他TetR家族蛋白已知结构中的相应距离长得多。此外，二聚体中的亚基具有不同的构象状态，导致DNA结合结构域和调节结构域的相对位置不同。在其他TetR调节因子中也观察到类似的构象修饰，并且是由配体结合引起的。这些研究提供了关于SCO0520分子的灵活性及其假定生物学功能的信息。
