Department of Internal and Parasitic Diseases, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Pl. Grunwaldzki 47, Poland.
Exp Parasitol. 2011 Aug;128(4):419-26. doi: 10.1016/j.exppara.2011.05.019. Epub 2011 May 24.
This study was carried out to determine the influence of short chain fatty acids (SCFA) on spleen and mesenteric lymph node lymphocyte proliferation, goblet cells and apoptosis in the mouse small intestine during invasion by Trichinella spiralis. BALB/c mice were infected with 250 larvae of T. spiralis. An SCFA water solution containing acetic, propionic and butyric acids (30:15:20 mM) was administered orally starting 5 days before infection and ending 20 days post infection (dpi). Fragments of the jejunum were collected by dissection 7 and 10 dpi, and were examined for apoptotic cells in the lamina propria of the intestinal mucosa, and for goblet cells. The proliferation index of the cultured spleen and mesenteric lymph node lymphocytes with MTT test was also determined. The orally administered SCFA solution decreased the proliferation of mesenteric lymph node lymphocytes in the mice infected with T. spiralis at both examination times, but did not influence the proliferative activity of the spleen cells. Seven dpi, both in the spleen and mesenteric lymph nodes, the highest proliferation index of concanavalin A (Con A)-stimulated lymphocytes was found in the group of uninfected animals receiving SCFA animals. This tendency could still be seen 10 dpi in the mesenteric lymph nodes but not in the spleen, where the proliferation index in this group had significantly decreased. In vitro studies revealed, that butyric and propionic acids added to the cell cultures suppressed the proliferation of Con A-stimulated mesenteric lymph nodes and spleen lymphocytes taken from uninfected and T. spiralis-infected mice. Acetic acid stimulated proliferation of splenocytes taken from uninfected mice but did not affect lymphocyte proliferation in mesenteric lymph nodes from uninfected or infected mice. Orally administered SCFA increased the number of goblet cells found in the epithelium of the jejunum 7 dpi, but this number had decreased 10 dpi. The number of apoptotic cells in the lamina propria of the intestinal mucosa of animals infected with the T. spiralis and receiving SCFA was also lower, particularly 10 dpi. The above results show that SCFA can participate in the immune response during the course of trichinellosis in mice.
本研究旨在探讨短链脂肪酸（SCFA）对旋毛虫感染小鼠脾脏和肠系膜淋巴结淋巴细胞增殖、杯状细胞和空肠细胞凋亡的影响。BALB/c 小鼠感染 250 条旋毛虫幼虫。在感染前 5 天至感染后 20 天（dpi），给予含有乙酸、丙酸和丁酸（30:15:20mM）的 SCFA 水溶液进行口服治疗。在感染后 7 和 10 dpi 通过解剖采集空肠片段，并在肠黏膜固有层中检测凋亡细胞和杯状细胞。用 MTT 试验测定培养的脾脏和肠系膜淋巴结淋巴细胞的增殖指数。口服 SCFA 溶液降低了感染旋毛虫的小鼠肠系膜淋巴结淋巴细胞的增殖，在两次检查时均如此，但对脾细胞的增殖活性没有影响。7dpi 时，在未感染和接受 SCFA 治疗的动物的脾脏和肠系膜淋巴结中，刀豆球蛋白 A（Con A）刺激的淋巴细胞增殖指数最高。这种趋势在肠系膜淋巴结中仍可见于 10dpi，但在脾中则不可见，该组的增殖指数显著降低。体外研究表明，添加到细胞培养物中的丁酸和丙酸抑制了来自未感染和感染旋毛虫的小鼠的 Con A 刺激的肠系膜淋巴结和脾淋巴细胞的增殖。乙酸刺激来自未感染小鼠的脾细胞增殖，但不影响来自未感染或感染小鼠的肠系膜淋巴结淋巴细胞增殖。口服 SCFA 增加了感染旋毛虫的小鼠空肠上皮中杯状细胞的数量，这一数量在 10dpi 时有所下降。接受 SCFA 的动物的肠黏膜固有层中凋亡细胞的数量也减少，特别是在 10dpi 时。上述结果表明，SCFA 可以参与感染旋毛虫的小鼠的免疫反应过程。
