University of Lyon, Physiological and Pathological Functions of the Cutaneous Barrier, Laboratory for R&D in Industrial Pharmaceutical Technologies, Lyon, France.
Eur J Dermatol. 2011 May;21 Suppl 2:52-62. doi: 10.1684/ejd.2011.1270.
Often presented as metabolism byproducts, reactive oxygen species are linked to detrimental effects such as chronic wound, mutagenesis, cancer and skin ageing. However, recent in vitro and in vivo observations suggest that ROS, and mainly hydrogen peroxide, interfere with cell signaling acting like second messenger and inducing adaptive responses. This is particularly observed in skin wound healing where cells are exposed to H₂O₂ following injury. In this study, we developed and characterized an innovative formulation producing H₂O₂ at low concentrations, in order to mimic physiological inflammation phase. Then, this pro-oxidative formulation (CAM-GOx) was assayed in vitro on keratinocytes cell culture, compared to the blank formulation (CAM) and the anti-oxidative formulation (CAM-CAT) to assess whether oxidative stress was implied or not in cellular responses.
活性氧（ROS）通常被认为是新陈代谢的副产物，与慢性伤口、突变、癌症和皮肤衰老等有害作用有关。然而，最近的体外和体内观察表明，ROS，主要是过氧化氢，通过充当第二信使和诱导适应性反应来干扰细胞信号转导。这在皮肤伤口愈合中尤为明显，细胞在受伤后会暴露于 H₂O₂。在这项研究中，我们开发并表征了一种创新的制剂，可在低浓度下产生 H₂O₂，以模拟生理炎症阶段。然后，在体外的角质形成细胞培养物上测试了这种促氧化制剂（CAM-GOx），并与空白制剂（CAM）和抗氧化制剂（CAM-CAT）进行了比较，以评估细胞反应中是否存在氧化应激。
