Isogenica Ltd., The Mansion, Chesterford Research Park, Little Chesterford, Essex, UK.
Brief Funct Genomics. 2011 May;10(3):125-34. doi: 10.1093/bfgp/elr010.
The presentation of recombinant peptide libraries linked to their coding sequence can be referred to as 'peptide display'. Phage display is the most widely practiced peptide display technology but more recent alternatives such as CIS display, ribosome display and mRNA display offer advantages over phage for speed, library size and the display of unnatural amino acids. These have provided researchers with tools to address some of the failings of peptides such as their low affinity, low stability and inability to cross biological membranes. In this review, we assess some of the recent advances in peptide display and its application.
与编码序列相连的重组肽文库的呈现方式可以称为“肽展示”。噬菌体展示是应用最广泛的肽展示技术，但最近出现的替代技术，如 CIS 展示、核糖体展示和 mRNA 展示，在速度、文库大小和非天然氨基酸展示方面优于噬菌体。这些技术为研究人员提供了工具，可以解决肽的一些缺陷，如亲和力低、稳定性差和无法穿过生物膜。在这篇综述中，我们评估了肽展示及其应用的一些最新进展。
