志愿者挑战表达肠致病性大肠杆菌肠定植因子菌毛 CS17 和 CS19
Volunteer challenge with enterotoxigenic Escherichia coli that express intestinal colonization factor fimbriae CS17 and CS19.
机构信息
Department of Medicine, Johns Hopkins University, Johns Hopkins Bayview Medical Center, MD, USA.
出版信息
J Infect Dis. 2011 Jul 1;204(1):60-4. doi: 10.1093/infdis/jir220.
Abstract
Human challenges with enterotoxigenic Escherichia coli (ETEC) have broadened our understanding of this important enteropathogen. We report findings from the first challenge studies using ETEC-expressing colonization factor fimbria CS17 and CS19. LSN03-016011/A (LT, CS17) elicited a dose-dependent effect, with the upper dose (6 × 10(9) organisms) causing diarrhea in 88% of recipients. WS0115A (LTSTp, CS19) also showed a dose response, with a 44% diarrhea rate at 9 × 10(9) organisms. Both strains elicited homologous antifimbrial and anti-LT antibody seroconversion. These studies establish the relative pathogenicity of ETEC expressing newer class 5 fimbriae and suggest suitability of the LT|CS17-ETEC challenge model for interventional trials.
摘要
人类感染肠产毒性大肠杆菌（ETEC）的挑战拓宽了我们对这种重要肠病原体的认识。我们报告了首次使用表达定植因子纤毛 CS17 和 CS19 的 ETEC 进行挑战研究的结果。LSN03-016011/A（LT、CS17）引起了剂量依赖性效应，高剂量（6×10^9 个生物体）导致 88%的受者腹泻。WS0115A（LTSTp、CS19）也表现出剂量反应，在 9×10^9 个生物体时腹泻率为 44%。两种菌株均引起同源性抗纤毛和抗 LT 抗体血清转化率。这些研究确定了表达新型 5 类纤毛的 ETEC 的相对致病性，并表明 LT|CS17-ETEC 挑战模型适合干预试验。
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