霍乱毒素在T细胞受体介导的激活过程中对辅助性T细胞1型和2型进行区分：环磷酸腺苷在T细胞增殖中的作用
Cholera toxin discriminates between T helper 1 and 2 cells in T cell receptor-mediated activation: role of cAMP in T cell proliferation.
作者信息
Muñoz E, Zubiaga A M, Merrow M, Sauter N P, Huber B T
机构信息
Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts 02111.
出版信息
J Exp Med. 1990 Jul 1;172(1):95-103. doi: 10.1084/jem.172.1.95.
Abstract
CD4+ T helper (Th) clones can be divided into interleukin 2 (IL-2)-secreting Th1 and IL-4-secreting Th2 cells. We show in the present report that these two Th subsets have different activation requirements for lymphokine production and proliferation: namely, cholera toxin (CT) as well as forskolin inhibit T cell receptor (TCR)-mediated IL-2 production and proliferation in Th1 cells, while the same reagents fail to block IL-4 production and proliferation in Th2 cells. In addition, CT and forskolin differentially influence the proto-oncogene mRNA expression in Th1 vs. Th2 cells after stimulation with Con A. Since both reagents lead to elevated levels of intracellular cAMP, it is likely that Th1 and Th2 cells differ in their sensitivity to an increase in cAMP. Our results indicate that the two Th subsets use different transmission signal pathways upon TCR-mediated activation.
摘要
CD4 + T辅助（Th）克隆可分为分泌白细胞介素2（IL-2）的Th1细胞和分泌IL-4的Th2细胞。我们在本报告中表明，这两个Th亚群对淋巴因子产生和增殖具有不同的激活要求：即霍乱毒素（CT）以及福斯高林抑制Th1细胞中T细胞受体（TCR）介导的IL-2产生和增殖，而相同试剂不能阻断Th2细胞中的IL-4产生和增殖。此外，在经伴刀豆球蛋白A刺激后，CT和福斯高林对Th1细胞与Th2细胞中原癌基因mRNA表达有不同影响。由于这两种试剂均导致细胞内cAMP水平升高，Th1细胞和Th2细胞对cAMP增加的敏感性可能不同。我们的结果表明，两个Th亚群在TCR介导的激活过程中使用不同的信号传导途径。
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Cholera toxin discriminates between T helper 1 and 2 cells in T cell receptor-mediated activation: role of cAMP in T cell proliferation.霍乱毒素在T细胞受体介导的激活过程中对辅助性T细胞1型和2型进行区分：环磷酸腺苷在T细胞增殖中的作用
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