Bardi Antonella, Cavazzini Francesco, Rigolin Gian Matteo, Tammiso Elisa, Volta Eleonora, Pezzolo Elisa, Formigaro Luca, Sofritti Olga, Daghia Giulia, Ambrosio Cristina, Rizzotto Lara, Abass Awad E, D'Auria Fiorella, Musto Pellegrino, Cuneo Antonio
Section of Haematology, Department of Bio-Medical Sciences and Advanced Therapies, University of Ferrara, Ferrara, Italy.
J Biomed Biotechnol. 2011;2011:691493. doi: 10.1155/2011/691493. Epub 2011 May 21.
To compare the efficiency of novel mitogenic agents and traditional mitosis inductors, 18 patients with splenic marginal zone lymphoma (SMZL) were studied. Three cultures using oligodeoxynucleotide (ODN) plus interleukin-2 (IL-2), or TPA, or LPS were setup in each patient. Seventeen/18 cases with ODN + IL2 had moderate/good proliferation (94, 4%) as compared with 10/18 cases with TPA and LPS (55%) (P = .015); 14/18 (77, 7%) cases with ODN + IL2 had sufficient good quality of banding as compared with 8/18 cases (44, 4%) with TPA and LPS. The karyotype could be defined from ODN + IL2-stimulated cultures in all 18 patients, 14 of whom (77, 7%) had a cytogenetic aberration, whereas clonal aberrations could be documented in 9 and in 3 cases by stimulation with LPS and TPA, respectively. Recurrent chromosome aberrations in our series were represented by aberrations of chromosome 14q in 5 patients, by trisomy 12 and 7q deletion in 4 cases each, and by abnormalities involving 11q and 13q in two cases each. These findings show that stimulation with ODN + IL2 offers more mitotic figures of better quality and results in an increased rate of clonal aberrations in SMZL, making this method ideal for prospective studies aiming at the definition of the prognostic impact of cytogenetic aberrations in this disorder.
为比较新型促有丝分裂剂与传统有丝分裂诱导剂的效率，对18例脾边缘区淋巴瘤（SMZL）患者进行了研究。为每位患者设置了三种培养体系，分别使用寡脱氧核苷酸（ODN）加白细胞介素-2（IL-2）、佛波酯（TPA）或脂多糖（LPS）。与使用TPA和LPS的10/18例患者（55%）相比，17/18例使用ODN + IL2的患者具有中度/良好增殖（94.4%）（P = 0.015）；与使用TPA和LPS的8/18例患者（44.4%）相比，14/18例（77.7%）使用ODN + IL2的患者具有足够良好的带型质量。在所有18例患者中，均可从ODN + IL2刺激的培养物中确定核型，其中14例（77.7%）存在细胞遗传学异常，而通过LPS和TPA刺激，分别有9例和3例可记录到克隆性异常。在我们的系列研究中，复发性染色体异常表现为：5例患者存在14q染色体异常，4例患者各有12号染色体三体和7q缺失，2例患者各有涉及11q和13q的异常。这些发现表明，ODN + IL2刺激可提供更多质量更好的有丝分裂图像，并导致SMZL中克隆性异常率增加，使得该方法非常适合旨在明确细胞遗传学异常对该疾病预后影响的前瞻性研究。
