Draper R K, Goda Y, Brodsky F M, Pfeffer S R
Department of Biochemistry, Stanford University School of Medicine, CA 94305-5307.
Science. 1990 Jun 22;248(4962):1539-41. doi: 10.1126/science.2163108.
Mannose 6-phosphate receptors carry newly synthesized lysosomal enzymes from the trans Golgi network (TGN) to prelysosomes and then return to the TGN to carry out another round of lysosomal enzyme delivery. Although clathrin-coated vesicles mediate the export of mannose 6-phosphate receptors from the TGN, nothing is known about the transport vesicles used to carry these receptors back to the TGN. Two different in vitro assays used in this study show that an antibody that interferes with clathrin assembly blocks receptor-mediated endocytosis of transferrin, but has no effect on the recycling of the 300-kilodalton mannose 6-phosphate receptor from prelysosomes to the TGN. These results suggest that the transport of mannose 6-phosphate receptors from prelysosomes to the TGN does not involve clathrin.
甘露糖6-磷酸受体将新合成的溶酶体酶从反式高尔基体网络（TGN）转运至前溶酶体，然后返回TGN以进行另一轮溶酶体酶的递送。虽然网格蛋白包被的囊泡介导甘露糖6-磷酸受体从TGN输出，但对于用于将这些受体运回TGN的转运囊泡却一无所知。本研究中使用的两种不同的体外测定表明，一种干扰网格蛋白组装的抗体可阻断转铁蛋白的受体介导的内吞作用，但对300千道尔顿甘露糖6-磷酸受体从前溶酶体到TGN的再循环没有影响。这些结果表明，甘露糖6-磷酸受体从前溶酶体到TGN的转运不涉及网格蛋白。
