Haginaka J, Wakai J
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Anal Chem. 1990 May 15;62(10):997-1000. doi: 10.1021/ac00209a006.
A new beta-cyclodextrin (CD) bonded silica has been designed for direct injection analysis of drug enantiomers in serum for liquid chromatography. The new beta-CD bonded silica is synthesized by three steps: introduction of a 3-glycidoxypropyl phase, introduction of a beta-CD carbamate-bonded phase, and hydrolysis of the oxirane ring to a diol phase. Although the recovery of serum proteins from only a beta-CD bonded silica (having no diol phase) was poor, they were completely recovered from a mixed functional silica having beta-CD and diol phases. Direct injection analysis of drug enantiomers in serum can be attained with the mixed functional silica support over the eluent pH range of 3-7. The recovery of racemic drugs from serum was almost 100%.
一种新型的β-环糊精（CD）键合硅胶已被设计用于液相色谱法直接进样分析血清中的药物对映体。这种新型的β-环糊精键合硅胶通过三步合成：引入3-环氧丙氧基丙基相、引入β-环糊精氨基甲酸酯键合相以及将环氧乙烷环水解为二醇相。尽管仅从β-环糊精键合硅胶（无二醇相）中回收血清蛋白的效果不佳，但从具有β-环糊精和二醇相的混合功能硅胶中可以完全回收血清蛋白。在3-7的洗脱液pH范围内，使用混合功能硅胶载体可实现血清中药物对映体的直接进样分析。外消旋药物从血清中的回收率几乎为100%。
