Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, 259 Wen-Hwa first Road, Kwei-Shan, Tao-Yuan 333, Taiwan.
J Biosci Bioeng. 2011 Sep;112(3):219-24. doi: 10.1016/j.jbiosc.2011.05.004. Epub 2011 May 31.
A novel magnetic adsorbent was prepared by covalently binding lysine onto the surface of nanoparticles via a carbodiimide coupling method. The adsorption of plasminogen onto surface-modified magnetic nanoparticles from human plasma was studied in a batch system. Surface modifications of particles were characterized using Fourier-transformed infrared spectra, transmission electron micrography, and ninhydrin assay. The maximum weight ratio of lysine to the superparamagnetic particles was 30 μmol/mg of particles. Effects of pH and temperature on plasminogen adsorption by the magnetic particles were evaluated. Desorption of plasminogen from the magnetic adsorbent was investigated using aminocaproic acid, a lysine analogue. Using a buffer composed by aminocaproic acid or lysine, plasminogen on the magnetic nanoparticles could be eluted. Overall, the results demonstrated that the lysine-coated magnetic adsorbent increased the efficiency and speed of recovery of plasminogen from human plasma.
通过碳二亚胺偶联法将赖氨酸共价结合到纳米粒子表面，制备了一种新型磁性吸附剂。在批处理系统中研究了人血浆中纤维蛋白溶酶原吸附到表面修饰的磁性纳米粒子上的情况。使用傅里叶变换红外光谱、透射电子显微镜和茚三酮试验对颗粒的表面修饰进行了表征。赖氨酸与超顺磁颗粒的最大重量比为 30μmol/mg 颗粒。评估了 pH 值和温度对磁性颗粒吸附纤维蛋白溶酶原的影响。使用赖氨酸类似物氨基己酸研究了从磁性吸附剂上解吸纤维蛋白溶酶原的情况。使用由氨基己酸或赖氨酸组成的缓冲液，可以从磁性纳米颗粒上洗脱纤维蛋白溶酶原。总的来说，结果表明，赖氨酸涂层的磁性吸附剂提高了从人血浆中回收纤维蛋白溶酶原的效率和速度。
