牛蛙蝌蚪尾巴在自发变态和三碘甲状腺原氨酸诱导变态过程中超氧阴离子产生速率的变化。

Changes in the rate of production of superoxide anion in tails of Rana catesbeiana tadpoles during spontaneous and triiodothyronine-induced metamorphosis.

Little G H, Flores A

Department of Biochemistry and Molecular Biology, Texas Tech University Health Sciences Center, Lubbock 79430.

Comp Biochem Physiol B. 1990;96(2):315-8. doi: 10.1016/0305-0491(90)90380-c.

Abstract

The time course of changes in the rate of production of superoxide anion (O2) was compared with that of beta-glucuronidase in tails of Rana catesbeiana tadpoles during spontaneous and triiodothyronine (T3)-induced metamorphosis. 2. Superoxide production increases in tadpole tail tissue undergoing regression in vivo during spontaneous metamorphosis and in response to T3 treatment. 3. The specific activity of beta-glucuronidase rises three-fold relative to control levels in tadpoles treated with T3 in vivo. 4. The time of onset of the rise in beta-glucuronidase activity precedes the onset of tissue regression and the onset of the increase in superoxide production precedes the rise in beta-glucuronidase activity.

摘要

比较了牛蛙蝌蚪尾巴在自发变态和三碘甲状腺原氨酸（T3）诱导变态过程中超氧阴离子（O2）产生速率的变化时间进程与β-葡萄糖醛酸酶的变化时间进程。2. 在自发变态期间以及对T3处理作出反应时，正在体内发生退化的蝌蚪尾巴组织中超氧产生增加。3. 体内用T3处理的蝌蚪中，β-葡萄糖醛酸酶的比活性相对于对照水平升高了三倍。4. β-葡萄糖醛酸酶活性升高的起始时间先于组织退化的起始时间，超氧产生增加的起始时间先于β-葡萄糖醛酸酶活性的升高。