解析 ABA 在水分亏缺诱导的紫花苜蓿幼苗氮代谢调节中的作用。
Unraveling the involvement of ABA in the water deficit-induced modulation of nitrogen metabolism in Medicago truncatula seedlings.
机构信息
University of Angers, Seed Molecular Physiology, Angers, France.
出版信息
Plant Signal Behav. 2011 Jul;6(7):1074-6. doi: 10.4161/psb.6.7.15653.
Effects of water deficit and/or abscisic acid (ABA) were investigated on early seedling growth of Medicago truncatula, and on glutamate metabolism under dark conditions. Water deficit (simulated by polyethylene glycol, PEG), ABA and their combination resulted in a reduction in growth rate of the embryo axis, and also in a synergistic increase of free amino acid (AA) content. However, the inhibition of water uptake retention induced by water deficit seemed to occur in an ABA-independent manner. Expression of several genes involved in glutamate metabolism was induced during water deficit, whereas ABA, in combination or not with PEG, repressed them. The only exception came from a gene encoding 1-pyrroline-5-carboxylate synthetase (P5CS) which appeared to be induced in an ABA-dependent manner under water deficit. Our results demonstrate clearly the involvement of an ABA-dependent and an ABA-independent regulatory system, governing growth and glutamate metabolism under water deficit.
研究了水分亏缺和/或脱落酸（ABA）对蒺藜苜蓿幼苗早期生长和黑暗条件下谷氨酸代谢的影响。水分亏缺（通过聚乙二醇，PEG 模拟）、ABA 及其组合导致胚胎轴生长速率降低，并协同增加游离氨基酸（AA）含量。然而，水分亏缺引起的水分吸收保留的抑制似乎以 ABA 非依赖性方式发生。参与谷氨酸代谢的几个基因的表达在水分亏缺期间被诱导，而 ABA 结合或不结合 PEG 则抑制它们。唯一的例外来自编码 1-吡咯啉-5-羧酸合成酶（P5CS）的基因，该基因在水分亏缺下似乎以 ABA 依赖性方式被诱导。我们的结果清楚地表明，ABA 依赖性和 ABA 非依赖性调节系统参与了水分亏缺下的生长和谷氨酸代谢的调控。
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