建立可逆性胆汁淤积大鼠模型。
Creation of reversible cholestatic rat model.
作者信息
Subhas Gokulakkrishna, Bhullar Jasneet, Mittal Vijay K, Jacobs Michael J
机构信息
Department of General Surgery, Providence Hospital and Medical Centers, RI, USA.
出版信息
J Vis Exp. 2011 May 21(51):2692. doi: 10.3791/2692.
Cholestasis is a clinical condition commonly encountered by both surgeons and gastroenterologists. Cholestasis can cause various physiological changes and affect the nutritional status and surgical outcomes. Study of the pathophysiological changes occurring in the liver and other organs is of importance. Various studies have been done in cholestatic rat models. We used a reversible cholestatic rat model in our recent study looking at the role of methylprednisolone in the ischemia reperfusion injury. Various techniques for creation of a reversible cholestatic model have been described. Creation of a reversible cholestatic rat model can be challenging in view of the smaller size and unique hepatopancreatobiliary anatomy in rats. This video article demonstrates the creation of a reversible cholestatic model. This model can be used in various studies, such as looking at the changes in nutritional, physiological, pathological, histological and immunological changes in the gastrointestinal tract. This model can also be used to see the effects of cholestasis and various therapeutic interventions on major hepatic surgeries.
胆汁淤积是外科医生和胃肠病学家都常遇到的一种临床病症。胆汁淤积会引发各种生理变化，并影响营养状况和手术效果。研究肝脏及其他器官中发生的病理生理变化具有重要意义。针对胆汁淤积大鼠模型已开展了多项研究。在我们最近一项研究甲基强的松龙在缺血再灌注损伤中作用的实验里，我们使用了一种可逆性胆汁淤积大鼠模型。已描述了多种构建可逆性胆汁淤积模型的技术。鉴于大鼠体型较小且肝胰胆解剖结构独特，构建可逆性胆汁淤积大鼠模型可能具有挑战性。这篇视频文章展示了可逆性胆汁淤积模型的构建过程。该模型可用于各种研究，比如观察胃肠道营养、生理、病理、组织学及免疫学变化。此模型还可用于观察胆汁淤积及各种治疗干预对 major hepatic surgeries（此处可能有误，推测是major hepatic surgeries，可译为“大型肝脏手术”）的影响。
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