Damien C J, Parsons J R, Benedict J J, Weisman D S
Laboratories for Orthopaedic Research, UMDNJ/New Jersey Medical School, Newark 07103.
J Biomed Mater Res. 1990 Jun;24(6):639-54. doi: 10.1002/jbm.820240602.
In the search for a suitable bone graft substitute, a study was conducted using a material that combined a proven osteoconductive composite, hydroxyapatite-calcium sulfate (HA/CS), with an osteoinductive factor, bovine osteogenic factor (OF). The initial study demonstrated the osteoinductive potential of OF in the rabbit muscle model. Once satisfied that the OF was active, it was added to the HA/CS composite and placed in 8-mm trephine defects in the rabbit cranium. This HA/CS/OF was directly compared to HA/CS augmented with a control protein, rabbit serum albumin (RSA). Animals were sacrificed at 4 and 8 weeks. Results demonstrated increased bone formation with the addition of the OF to the composite with bridging of the defects, in most cases, by 4 weeks. No bridging was seen, at this time period, in the other defects left unfilled or filled with HA/CS/RSA. Osteogenic factor, with the appropriate delivery system, can induce bone formation in the rabbit muscle. It may also increase the rate of bone formation at early time periods in a bony defect site when the delivery system is the osteoconductive composite HA/CS.
在寻找合适的骨移植替代物的过程中，进行了一项研究，使用了一种将经过验证的骨传导复合材料羟基磷灰石 - 硫酸钙（HA/CS）与骨诱导因子牛成骨因子（OF）相结合的材料。初步研究证明了OF在兔肌肉模型中的骨诱导潜力。一旦确定OF具有活性，就将其添加到HA/CS复合材料中，并置于兔颅骨的8毫米环锯缺损处。将这种HA/CS/OF与添加对照蛋白兔血清白蛋白（RSA）的HA/CS进行直接比较。在4周和8周时处死动物。结果表明，在复合材料中添加OF可增加骨形成，在大多数情况下，缺损在4周时实现桥接。在此时间段内，其他未填充或填充有HA/CS/RSA的缺损未见桥接。成骨因子与合适的递送系统相结合，可在兔肌肉中诱导骨形成。当递送系统为骨传导复合材料HA/CS时，它还可能在早期增加骨缺损部位的骨形成速率。
