核糖核酸聚合酶的亚基内核苷酸结合
Intrasubunit nucleotide binding in ribonucleic acid polymerase.
作者信息
Malcolm A D, Moffatt J R
出版信息
Biochem J. 1978 Oct 1;175(1):189-92. doi: 10.1042/bj1750189.
Abstract
- Periodate oxidation of the ribose ring was used to synthesize derivatives of nucleoside triphosphates. 2. These oxidized nucleoside triphosphates. 2. These oxidized nucleoside triphosphates are competitive inhibitors of RNA polymerase. 3. On incubation, together with NaBH4, these oxidized labelled nucleotides are covalently bound to Escherichia coli RNA polymerase. 4. Nucleoside triphosphate substrates decrease the extent of labelling. 5. A lysine residue in an alpha-subunit is labelled. 6. The significance of these results in relation to the location of the nucleotide-binding site is discussed.
摘要
- 核糖环的高碘酸盐氧化被用于合成核苷三磷酸的衍生物。2. 这些氧化的核苷三磷酸。2. 这些氧化的核苷三磷酸是RNA聚合酶的竞争性抑制剂。3. 与NaBH4一起温育时，这些氧化的标记核苷酸会与大肠杆菌RNA聚合酶共价结合。4. 核苷三磷酸底物会降低标记程度。5. α亚基中的一个赖氨酸残基被标记。6. 讨论了这些结果与核苷酸结合位点位置的相关性。
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3
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Biochemistry. 1969 Nov;8(11):4450-8. doi: 10.1021/bi00839a034.
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