Hickman J, McElduff A
Department of Endocrinology, Royal North Shore Hospital, St. Leonards, Australia.
Calcif Tissue Int. 1990 Jun;46(6):401-5. doi: 10.1007/BF02554971.
Pretreatment of the osteogenic sarcoma cell line UMR-106-01 with insulin results in sensitization to both parathyroid hormone (PTH) and isoproterenol. In insulin-pretreated cells, the two hormones cause a significantly greater cyclic AMP (cAMP) accumulation than in noninsulin-treated cells. In the presence of cholera toxin, which enhances cAMP production by these cells in both the basal and PTH-stimulated state, the effect of insulin is maintained. In the presence of pertussis toxin, which has no effect on basal cAMP accumulation but enhances both PTH and isoproterenol stimulation, insulin sensitization for both hormones is abolished. These data suggest that insulin sensitizes these cells to subsequent hormone stimulation by lessening the action of an inhibitory guanine nucleotide regulatory protein, possibly Gi.
用胰岛素对骨肉瘤细胞系UMR - 106 - 01进行预处理可使其对甲状旁腺激素（PTH）和异丙肾上腺素敏感。在胰岛素预处理的细胞中，这两种激素引起的环磷酸腺苷（cAMP）积累比未用胰岛素处理的细胞显著更多。在霍乱毒素存在的情况下，霍乱毒素在基础状态和PTH刺激状态下均增强这些细胞的cAMP产生，胰岛素的作用得以维持。在百日咳毒素存在的情况下，百日咳毒素对基础cAMP积累无影响，但增强PTH和异丙肾上腺素的刺激作用，对这两种激素的胰岛素敏感性均被消除。这些数据表明，胰岛素通过减弱抑制性鸟嘌呤核苷酸调节蛋白（可能是Gi）的作用，使这些细胞对随后的激素刺激敏感。
