Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest AF University, Yangling, Shaanxi China.
Genet Mol Biol. 2010 Apr;33(2):266-70. doi: 10.1590/S1415-47572010005000034. Epub 2010 Jun 1.
The association of IGF-I gene polymorphisms with certain traits in 708 individuals of two Chinese dairy-goat breeds (Guanzhong and Xinong Saanen) was investigated. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing methods were employed in screening for genetic variation. Two novel mutations were detected in the 5'-flanking region and in intron 4 of IGF-I gene, viz., g.1617 G > A and g.5752 G > C (accession D26119.2), respectively. The associations of the g.1617 G > A mutation with milk yield and the body size were not significant (p > 0.05). However, in the case of g.5752 G > C, Xinong Saanen dairy goats with the CG genotype presented longer bodies (p < 0.05). Chest circumference (p < 0.05) was larger in Guanzhong goats with the GG genotype. In Xinong Saanen dairy goats with the CC genotype, milk yields were significantly higher during the first and second lactations (p < 0.05). Hence, the g.5752 G > C mutation could facilitate association analysis and serve as a genetic marker for Chinese dairy-goat breeding and genetics.
研究了 IGF-I 基因多态性与关中奶山羊和新疆奶山羊两个中国奶山羊品种 708 个个体某些特征的关系。采用聚合酶链反应-单链构象多态性（PCR-SSCP）和 DNA 测序方法筛选遗传变异。在 IGF-I 基因的 5'侧翼区和内含子 4 中检测到两个新的突变，即 g.1617 G > A 和 g.5752 G > C（登录号 D26119.2）。g.1617 G > A 突变与产奶量和体型的关联不显著（p > 0.05）。然而，对于 g.5752 G > C，CG 基因型的新疆奶山羊具有更长的身体（p < 0.05）。GG 基因型的关中奶山羊胸围更大（p < 0.05）。在 CC 基因型的新疆奶山羊中，第一和第二泌乳期的产奶量显著更高（p < 0.05）。因此，g.5752 G > C 突变可以促进关联分析，并可作为中国奶山羊育种和遗传学的遗传标记。
