Heck A J, Derrick P J
Institute of Mass Spectrometry and Department of Chemistry, University of Warwick, Coventry CV4 7AL, UK.
Anal Chem. 1997 Sep 1;69(17):3603-7. doi: 10.1021/ac970254b.
Using electrospray ionization with a 9.4 T Fourier transform mass spectrometer, fragment ion spectra were acquired for a single isotopomer of doubly protonated bradykinin (molecular mass, 1059.6 Da). Correlated sweep excitation methods were applied to mass-select the single isotopomer (m/z = 530.8). Sustained off-resonance irradiation was used to activate and fragment the ions. The accuracy (in terms of m/z) in detection of the fragment ions was on average 1.2 ppm, making the assignments unambiguous. The methods employed would be generally applicable to ions in the mass range of approximately 50 Da to 50 kDa.
使用配备9.4 T傅里叶变换质谱仪的电喷雾电离技术，对双质子化缓激肽的单一同位素异构体（分子量为1059.6 Da）采集碎片离子光谱。采用相关扫描激发方法对单一同位素异构体（m/z = 530.8）进行质量选择。使用持续非共振辐照来激活离子并使其碎片化。碎片离子检测的（m/z）准确度平均为1.2 ppm，使得归属明确无误。所采用的方法一般适用于质量范围约为50 Da至50 kDa的离子。
