Iizasa T, Yamaguchi Y, Tagawa M, Saito H, Fujisawa T, Kato K, Taniguchi M
Department of Surgery, School of Medicine, Chiba University, Japan.
Hybridoma. 1990 Jun;9(3):211-9. doi: 10.1089/hyb.1990.9.211.
Mouse-human heterohybridoma (3H12) producing human antibody was established by fusing P3/X63-Ag-U1 (P3U1) myeloma cells with lymphocytes from a patient of small cell lung carcinoma (SCLC). This monoclonal antibody reacts to lung cancer cells, especially SCLC, but not to adenocarcinoma or squamous cell carcinoma cells. It does not show any reactivities to other tumors or normal cells so far examined. An immunoprecipitation experiment with this antibody revealed that the antigen on SCLC was a single chain moiety of 150 kilodaltons (Kd). Judging from the cell type reactivity and molecular size of the antigen, this monoclonal antibody appears to detect a new tumor-associated antigen on human SCLC.
通过将P3/X63-Ag-U1（P3U1）骨髓瘤细胞与一名小细胞肺癌（SCLC）患者的淋巴细胞融合，建立了产生人抗体的小鼠-人杂交瘤（3H12）。这种单克隆抗体与肺癌细胞，特别是小细胞肺癌细胞发生反应，但不与腺癌或鳞状细胞癌细胞发生反应。到目前为止，它对所检测的其他肿瘤或正常细胞没有任何反应性。用这种抗体进行的免疫沉淀实验表明，小细胞肺癌上的抗原是一个150千道尔顿（Kd）的单链部分。从抗原的细胞类型反应性和分子大小判断，这种单克隆抗体似乎检测到了人小细胞肺癌上一种新的肿瘤相关抗原。
