Elliston J F, Tsai S Y, O'Malley B W, Tsai M J
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
J Biol Chem. 1990 Jul 15;265(20):11517-21.
We have constructed novel chimeric receptors consisting of the activation region of the herpes simplex virus transcription factor VP16 inserted into the amino-terminal region of the human estrogen receptor containing or lacking the hormone-binding region. By gene transfer into mammalian cells, these chimeric receptors behave in a hormone-dependent or hormone-independent manner, respectively, and are more efficient activators of gene expression than wild-type estrogen receptor. These studies indicate that a potent activation region from a viral transcription factor can be placed under hormonal control when introduced into a steroid receptor molecule and can enhance the receptor's potency (approximately 10-fold) in activating specific gene expression. It is likely that such chimeric molecules could be designed to increase selected target gene responses to any intracellular receptor in the course of cellular transfection, transformation, or transgenic animal experiments.
我们构建了新型嵌合受体，其由插入到人雌激素受体氨基末端区域（含或不含激素结合区域）的单纯疱疹病毒转录因子VP16的激活区域组成。通过基因转移到哺乳动物细胞中，这些嵌合受体分别以激素依赖性或激素非依赖性方式发挥作用，并且比野生型雌激素受体更有效地激活基因表达。这些研究表明，当将病毒转录因子的有效激活区域引入类固醇受体分子时，可以将其置于激素控制之下，并可增强受体激活特定基因表达的能力（约10倍）。很可能可以设计这样的嵌合分子，以在细胞转染、转化或转基因动物实验过程中增加选定靶基因对任何细胞内受体的反应。
