University of Osnabrück, Faculty of Biology and Chemistry, Department of Animal Physiology, Barbarastrasse 11, 49076 Osnabrück, Germany.
Eur J Cell Biol. 2011 Sep;90(9):688-95. doi: 10.1016/j.ejcb.2011.04.009. Epub 2011 Jun 2.
V-ATPases in eukaryotes are heteromultimeric, H(+)-transporting proteins. They are localized in a multitude of different membranes and energize many different transport processes. Unique features of V-ATPases are, on the one hand, their ability to regulate enzymatic and ion transporting activity by the reversible dissociation of the catalytic V(1) complex from the membrane bound proton translocating V(0) complex and, on the other hand, their high sensitivity to specific macrolides such as bafilomycin and concanamycin from streptomycetes or archazolid and apicularen from myxomycetes. Both features require distinct intramolecular as well as intermolecular interactions. Here we will summarize our own results together with newer developments in both of these research areas.
真核生物中的 V-ATPases 是异源多聚体的 H(+)转运蛋白。它们定位于多种不同的膜中，并为许多不同的运输过程提供能量。V-ATPases 的独特特征一方面是它们能够通过可逆地将催化 V(1)复合物从膜结合的质子转运 V(0)复合物中解离来调节酶和离子转运活性，另一方面是它们对来自链霉菌的特定大环内酯类物质（如巴弗洛霉素和康纳霉素）以及粘菌的 archazolid 和 apicularen 的高度敏感性。这两个特征都需要独特的分子内和分子间相互作用。在这里，我们将总结我们自己的结果以及这两个研究领域的最新进展。
