Department Center for Health and Biological Sciences, Pontifical Catholic University of Parana (PUC-PR), Brazil.
Eur J Obstet Gynecol Reprod Biol. 2011 Oct;158(2):298-304. doi: 10.1016/j.ejogrb.2011.05.004.
To study the effect of bone marrow derived-mononuclear stem cells transplantation in the growth, VEGF-R and TNF-alpha expression of surgically induced endometriosis in an experimental model.
This is an experimental study conducted in the Center for Health and Biological Sciences at the Pontifical Catholic University of Parana, Brazil. Endometriotic implants were surgically induced in 120 female Wistar rats. The animals with viable endometrial implant (larger than 25 mm(2)) were randomically divided into 3 groups to receive an intraperitoneal injection of 0.2 cc of saline solution (C group; n=30), a subcutaneous injection of 1mg/kg of leuprolide (L group; n=34), or an intraperitoneal injection of 5×10(6) bone marrow derived-mononuclear stem cells (SC group; n=36). They were sacrificed after 21 days to assess the implants' size and the tissue expression of vascular endothelial growth factor receptor (VEGF-R) and tumor necrosis factor-alpha (TNF-alpha).
Treatment with leuprolide decreased the surface area of the endometriotic implant compared to the SC group and the C group. The absolute reduction in the surface area of the implant was 16.5mm, 0mm, and 0mm (p=0.007), respectively, and the percent reduction was 40.2%, 0%, and 0% (p=0.001). VEGF-R expression in the endometriotic implant decreased after treatment in the L and SC groups compared to the C group (409.6 μm(2) vs. 465 μm(2) vs. 920.9 μm(2), respectively; p=0.021). TNF-alpha expression also reduced in the L and SC groups compared to the C group (585.7 μm(2) vs. 549.3 μm(2) vs. 2402.1 μm(2), respectively; p<0.001).
Bone marrow derived-mononuclear stem cells transplantation decreased the expression of VEGF-R and TNF-alpha in the endometriotic implant but did not reduce the surface area of the lesion.
研究骨髓来源的单核干细胞移植对实验性子宫内膜异位症生长、VEGF-R 和 TNF-α表达的影响。
这是在巴西天主教大学健康与生物科学中心进行的一项实验研究。通过手术在 120 只雌性 Wistar 大鼠中诱导子宫内膜异位症。将具有活性子宫内膜植入物（大于 25mm²）的动物随机分为 3 组，分别接受 0.2cc 生理盐水（C 组；n=30）、1mg/kg 亮丙瑞林（L 组；n=34）或 5×10⁶ 骨髓来源的单核干细胞（SC 组；n=36）的腹腔内注射。21 天后处死动物，评估植入物的大小以及血管内皮生长因子受体（VEGF-R）和肿瘤坏死因子-α（TNF-α）的组织表达。
与 SC 组和 C 组相比，亮丙瑞林治疗降低了子宫内膜异位症植入物的表面积。植入物表面积的绝对减少分别为 16.5mm²、0mm² 和 0mm²（p=0.007），减少百分比分别为 40.2%、0%和 0%（p=0.001）。与 C 组相比，L 组和 SC 组子宫内膜异位症植入物中的 VEGF-R 表达在治疗后降低（分别为 409.6μm²、465μm² 和 920.9μm²；p=0.021）。与 C 组相比，L 组和 SC 组的 TNF-α表达也降低（分别为 585.7μm²、549.3μm² 和 2402.1μm²；p<0.001）。
骨髓来源的单核干细胞移植降低了子宫内膜异位症植入物中 VEGF-R 和 TNF-α的表达，但并未减少病变的表面积。
