从圆斑蝰（Daboia russelli siamensis）和尖吻蝮（Agkistrodon piscivorus leucostoma）毒液中分离丝氨酸蛋白酶的系统发育分析。
Phylogenetic analysis of serine proteases from Russell's viper (Daboia russelli siamensis) and Agkistrodon piscivorus leucostoma venom.
机构信息
Snake Bite and Venom Research Unit, Chula Medical Research Center, Faculty of Medicine, Chulalongkorn University, Rama IV Road, Patumwan District, Bangkok 10330, Thailand.
出版信息
Toxicon. 2011 Aug;58(2):168-78. doi: 10.1016/j.toxicon.2011.05.014. Epub 2011 May 27.
Abstract
Serine proteases are widely found in snake venoms. They have variety of functions including contributions to hemostasis. In this study, five serine proteases were cloned and characterized from two different cDNA libraries: factor V activator (RVV-V), alpha fibrinogenase (RVAF) and beta fibrinogenase (RVBF) from Russell's viper (Daboia russelli siamensis), and plasminogen activator (APL-PA) and protein C activator (APL-C) from Agkistrodon piscivorus leucostoma. The snake venom serine proteases were clustered in phylogenetic tree according to their functions. K(A)/K(S) values suggested that accelerated evolution has occurred in the mature protein coding regions in cDNAs of snake venom serine proteases.
摘要
丝氨酸蛋白酶广泛存在于蛇毒中。它们具有多种功能，包括对止血的贡献。在这项研究中，从两个不同的 cDNA 文库中克隆和表征了五种丝氨酸蛋白酶：来自罗素氏蝮蛇（Daboia russelli siamensis）的因子 V 激活剂（RVV-V）、α纤维蛋白原酶（RVAF）和β纤维蛋白原酶（RVBF），以及来自尖吻蝮（Agkistrodon piscivorus leucostoma）的纤溶酶原激活剂（APL-PA）和蛋白 C 激活剂（APL-C）。蛇毒丝氨酸蛋白酶根据其功能聚类在系统发育树中。K(A)/K(S) 值表明，蛇毒丝氨酸蛋白酶 cDNA 成熟蛋白编码区发生了加速进化。
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