Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan.
J Biotechnol. 2011 Jul 20;154(4):205-8. doi: 10.1016/j.jbiotec.2011.05.009. Epub 2011 May 27.
The piggyBac transposon has recently attracted attention as a tool for transgene integration in mammalian cells. However, previous studies involving piggyBac investigated only transposition from circular DNA, although some linear DNA vectors are used to transfect mammalian cells. In this study, we compared the transposition efficiency of piggyBac between linear and circular DNA. Colony counting assay, luciferase assay, and plasmid rescue assay showed that piggyBac transposon can transpose from linear DNA, but its efficiency is lower than the transposition efficiency from circular DNA. These results suggest that circular DNA is more suitable as donor vectors of piggyBac than linear DNA.
piggyBac 转座子最近作为一种在哺乳动物细胞中整合转基因的工具引起了关注。然而，以前涉及 piggyBac 的研究仅调查了环状 DNA 的转座，尽管一些线性 DNA 载体被用于转染哺乳动物细胞。在这项研究中，我们比较了线性和环状 DNA 中 piggyBac 的转座效率。集落计数分析、荧光素酶分析和质粒拯救分析表明，piggyBac 转座子可以从线性 DNA 中转座，但效率低于从环状 DNA 中转座的效率。这些结果表明，与线性 DNA 相比，环状 DNA 更适合作为 piggyBac 的供体载体。
