Ophthalmology Center, the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
Neurosci Lett. 2011 Jul 20;499(2):99-103. doi: 10.1016/j.neulet.2011.05.042. Epub 2011 May 26.
To explore the effect of leukemia inhibitory factor on corneal nerve regeneration in a rabbit model after laser in situ keratomileusis. Thirty five healthy New Zealand rabbits were divided into three groups for a 6-month observation, the blank control group, the control group, and the treatment group respectively. Laser in situ keratomileusis for myopia was performed on 30 rabbits (60 eyes in total) and then 1 μg/ml LIF eye drops were used four times a day on the left eyes as the treatment group, and the balanced salt solution (BSS) was used on the right eyes as the control group. Nerve regeneration was evaluated by counting the new regenerated nerves in golden chloride staining. The parameters for dry eye include Schirmer I test and tear break-up time were also examined. The number of regenerated nerve fibers in the treatment group was significantly higher than that in the control group at all time points except the 6th month after LASIK (P<0.05). The parameters for dry eye between two groups were compared at each postoperative time point and the results showed they were significantly higher in the LIF-treated group than in the BSS-control group at 2w, 1m, and 3m respectively. Leukemia inhibitory factor can effectively accelerate the corneal nerve regeneration of rabbit eyes after LASIK surgery and decrease the occurrence of dry eye symptoms.
探讨白血病抑制因子（LIF）对 LASIK 术后兔眼角膜神经再生的影响。
35 只健康新西兰大白兔，随机分为空白对照组、对照组和治疗组，每组 10 只，共 30 只眼。空白对照组不做任何处理，后两组兔眼行 LASIK 手术造模。其中 30 只兔（60 只眼）右眼滴 BSS 作为对照组，左眼滴 1μg/ml 的 LIF 眼液 4 次/d 作为治疗组，于术后 1d、1w、2w、1m、3m、6m 观察角膜神经再生情况。
氯化金染色后计数新生神经纤维数，术后 1w、2w、3m 时治疗组新生神经纤维计数明显多于对照组（P<0.05）。术后 2w、1m、3m 时治疗组 Schirmer I 试验、泪膜破裂时间（BUT）均优于对照组（P<0.05）。
LIF 能有效促进 LASIK 术后兔眼角膜神经的再生，减轻干眼症状。
