Biotechnology Division, Korea Forest Research Institute, Suwon, Republic of Korea.
Gene. 2011 Sep 1;483(1-2):43-8. doi: 10.1016/j.gene.2011.05.015. Epub 2011 May 27.
Water uptake across cell membranes is a principal requirement for plant growth at both the cellular and whole-plant levels; water movement through plant membranes is regulated by aquaporins (AQPs) or major intrinsic proteins (MIPs). We examined the expression characteristics of the poplar plasma membrane intrinsic protein 1 gene (PatPIP1), a type of MIP, which was isolated from a suspension cell cDNA library of Populus alba×P. tremula var. glandulosa. Examination of protoplasts expressing the p35S-PatPIP1::sGFP fusion protein revealed that the protein was localized in the plasma membrane. Northern blot analysis revealed that the gene was strongly expressed in poplar roots and leaves. Gene expression was inducible by abiotic factors including drought, salinity, cold temperatures and wounding, and also by plant hormones including gibberellic acid, jasmonic acid and salicylic acid. Since we found that the PatPIP1 gene was strongly expressed in response to mannitol, NaCl, jasmonic acid and wounding, we propose that PatPIP1 plays an essential role in the defense of plants against water stress.
跨细胞膜的水分吸收是细胞水平和整个植物水平植物生长的主要要求；水通过植物膜的运动受水通道蛋白（AQPs）或主要内在蛋白（MIPs）调节。我们研究了从银白杨×欧洲山杨杂种悬浮细胞 cDNA 文库中分离的 MIP 型杨树质膜内在蛋白 1 基因（PatPIP1）的表达特征。对表达 p35S-PatPIP1::sGFP 融合蛋白的原生质体的检测表明，该蛋白定位于质膜上。Northern blot 分析表明，该基因在杨树的根和叶中强烈表达。基因表达可被非生物因子（包括干旱、盐度、低温和创伤）以及植物激素（包括赤霉素、茉莉酸和水杨酸）诱导。由于我们发现 PatPIP1 基因在甘露醇、NaCl、茉莉酸和创伤的刺激下强烈表达，因此我们推测 PatPIP1 在植物抵御水分胁迫方面发挥着重要作用。
