Gynaecology and Obstetrics, Department of Nursing School, Goiás Federal University, Goiânia, Brazil.
Ann Diagn Pathol. 2011 Oct;15(5):318-22. doi: 10.1016/j.anndiagpath.2011.02.009.
The objective of the study was to identify mastocytosis in the chorionic epithelium of the uterine cervix in HIV-infected and non-HIV-infected women in autopsy specimens using histochemistry and immunohistochemistry techniques. Sixteen cervical tissue specimens were collected, of which 10 (62.50%) were from HIV-infected women. Histochemical and immunohistochemical techniques were used to evaluate mast cell density using Giemsa stain and anti-mast cell tryptase and anti-mast cell chymase antibodies, respectively. The study of the sheets and counting of mast cells with blue (Giemsa) or brown staining (anti-mast cell tryptase or chymase antibodies) were performed by 3 examiners, and 10 consecutive fields were examined under a light microscope at 400× magnification. A significant difference was found in mast cell density in the chorionic epithelium of the cervix in HIV-infected compared with non-HIV-infected women. The present study may contribute to the characterization of genital mucosa abnormalities and help better understand the potential role of mast cells in HIV-infected women.
本研究的目的是使用组织化学和免疫组织化学技术，在尸检标本中识别感染 HIV 和未感染 HIV 的女性宫颈绒毛膜上皮中的肥大细胞。收集了 16 份宫颈组织标本，其中 10 份（62.50%）来自 HIV 感染女性。使用吉姆萨染色和抗肥大细胞胰蛋白酶及抗肥大细胞糜酶抗体分别评估肥大细胞密度的组织化学和免疫组织化学技术。通过 3 名检查者对带蓝色（吉姆萨）或棕色染色（抗肥大细胞胰蛋白酶或糜酶抗体）的切片进行研究，并在 400×放大倍数的光学显微镜下检查 10 个连续的视野。发现感染 HIV 的女性与未感染 HIV 的女性宫颈绒毛膜上皮中的肥大细胞密度存在显著差异。本研究可能有助于描述生殖器黏膜异常，并有助于更好地理解肥大细胞在感染 HIV 的女性中的潜在作用。
