Zemel M B, Sowers J R, Shehin S, Walsh M F, Levy J
Division of Endocrinology and Hypertension, Wayne State University, Detroit, MI 48202.
Metabolism. 1990 Jul;39(7):704-8. doi: 10.1016/0026-0495(90)90104-k.
Recent data from our laboratory indicate that reduced membrane Ca-adenosine triphosphatase (ATPase) activity in non-insulin-dependent diabetics may be responsible for increases in intracellular calcium and, consequently, for elevated vascular resistance. Since obesity is frequently associated with hypertension, even before the development of overt diabetes, we evaluated blood pressure and erythrocyte cation levels and membrane Na/K-ATPase and Ca-ATPase in Zucker obese rats and their lean controls (n = 10 per group). Intra-arterial blood pressure, determined via a femoral cannula, demonstrated elevated systolic and diastolic pressure in the obese rats (P less than .05). There were no significant differences in Na/K-ATPase between groups, but there was a decrease in Ca-ATPase (P less than .01) in the obese rats and an increase in tissue and cellular calcium content (P less than .05). These data demonstrate a specific impairment in membrane Ca-ATPase activity in obese rats they may have caused the observed increase in cellular calcium and, consequently, increased blood pressure. These phenomena may result from impaired insulin activation of membrane Ca-ATPase in these insulin-resistant animals.
我们实验室最近的数据表明，非胰岛素依赖型糖尿病患者细胞膜钙 - 腺苷三磷酸酶（ATP酶）活性降低可能是细胞内钙增加的原因，进而导致血管阻力升高。由于肥胖常与高血压相关，甚至在显性糖尿病发生之前就如此，我们评估了 Zucker 肥胖大鼠及其瘦对照（每组n = 10）的血压、红细胞阳离子水平以及细胞膜钠/钾 - ATP酶和钙 - ATP酶。通过股动脉插管测定的动脉内血压显示，肥胖大鼠的收缩压和舒张压升高（P <.05）。两组之间钠/钾 - ATP酶无显著差异，但肥胖大鼠的钙 - ATP酶降低（P <.01），组织和细胞钙含量增加（P <.05）。这些数据表明肥胖大鼠细胞膜钙 - ATP酶活性存在特定损害，这可能导致了观察到的细胞内钙增加，进而导致血压升高。这些现象可能是由于这些胰岛素抵抗动物中胰岛素对细胞膜钙 - ATP酶的激活受损所致。
