Gevaudan M J, Bollet C, Mallet M N, Gulian G, Tissot-Dupont H, de Micco P
Laboratoire de Microbiologie Hygiène et Epidémiologie Hospitalière, Marseille.
Pathol Biol (Paris). 1990 May;38(5):413-9.
The effect of two new macrolides, azithromycin and roxithromycin used either alone or in combination with amikacin rifabutine and 1.25 (HO)2 vitamin D3 was examined in vitro. Macrophage monolayers infected with M. avium complex or M. xenopi were treated with antibiotics or 1.25 (OH)2 vitamin D3 by using different protocols: antibiotics or 1.25 (OH)2 vitamin D3 was added to the macrophage monolayers immediately after infection and released by washing out after 24 h; antibiotics or 1.25 (OH)2 vitamin D3 was replenished daily for 4 days; or infected macrophage monolayers were treated with antibiotics plus vitamin D3 for 4 consecutive days. Treatment for 24 h resulted in an inhibition of growth. After treating the macrophage monolayers with the four antibiotics alone or in combination for 4 consecutive days intracellular killing of mycobacteria was observed and the combinations were significantly more lethal than antibiotics alone. The mycobactericidal effect was enhanced when vitamin D3 was added to the culture.
对两种新型大环内酯类药物阿奇霉素和罗红霉素单独使用或与阿米卡星、利福布丁及1,25-(OH)₂维生素D₃联合使用的体外效果进行了研究。用不同方案将感染鸟分枝杆菌复合群或蟾分枝杆菌的巨噬细胞单层用抗生素或1,25-(OH)₂维生素D₃进行处理：感染后立即将抗生素或1,25-(OH)₂维生素D₃加入巨噬细胞单层，并在24小时后通过冲洗去除；连续4天每天补充抗生素或1,25-(OH)₂维生素D₃；或者感染的巨噬细胞单层连续4天用抗生素加维生素D₃进行处理。处理24小时导致生长受到抑制。在用四种抗生素单独或联合对巨噬细胞单层连续处理4天后，观察到细胞内对分枝杆菌的杀伤作用，且联合使用比单独使用抗生素的致死性显著更高。当向培养物中添加维生素D₃时，杀分枝杆菌作用增强。
