Department of Gynecology, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil.
J Clin Pathol. 2011 Oct;64(10):853-7. doi: 10.1136/jclinpath-2011-200103. Epub 2011 Jun 4.
Vulvar squamous-cell carcinoma (SCC) is a rare gynaecological cancer. Vulvar SCC has been shown to develop from vulvar intraepithelial neoplasias, which are related to lichen sclerosus (LS). Most studies to date have compared vulvar SCC with LS only morphologically, but no detailed molecular analysis has been performed. The objective was to compare claudin and p53 expression in these diseases and determine if there was any association with expression and vulvar SCC progression.
Immunohistochemical analysis was performed in order to determine expression of p53 and claudin 1, 2, 3, 4, 5, 7 and 11 in human vulvar tissue samples from LS, SCC and control patients.
Claudin 1, 2, 3, 4 and 5 were expressed comparably in the three groups. Claudin 7 and 11 expression was significantly decreased in LS and SCC samples compared with the control group. Expression of p53 was significantly increased in SCC and LS patient samples compared with the control group.
Claudin 7 and 11 were not expressed in LS and SCC. However, there was no significant difference in expression of any of the claudins between the LS and SCC samples. Furthermore, p53 expression is the highest in SCC patients and lowest in the control group. However, expression of p53 did not vary between samples from isolated LS and LS associated SCC patients, suggesting that increased p53 expression is not the determining factor in the progression of LS lesions to SCC.
外阴鳞状细胞癌（SCC）是一种罕见的妇科癌症。外阴 SCC 已被证明是从外阴上皮内瘤变发展而来的，而外阴上皮内瘤变与硬化性苔藓（LS）有关。迄今为止，大多数研究仅在外阴 SCC 与 LS 形态学上进行了比较，但尚未进行详细的分子分析。目的是比较这些疾病中 Claudin 和 p53 的表达，并确定其与外阴 SCC 进展之间是否存在关联。
进行免疫组织化学分析，以确定 LS、SCC 和对照组患者的人外阴组织样本中 p53 和 Claudin 1、2、3、4、5、7 和 11 的表达。
Claudin 1、2、3、4 和 5 在三组中的表达相似。Claudin 7 和 11 的表达在 LS 和 SCC 样本中与对照组相比明显降低。与对照组相比，SCC 和 LS 患者样本中的 p53 表达明显增加。
Claudin 7 和 11 在 LS 和 SCC 中不表达。然而，LS 和 SCC 样本之间任何 Claudin 的表达均无显着差异。此外，p53 表达在外阴 SCC 患者中最高，在对照组中最低。然而，孤立性 LS 和 LS 相关 SCC 患者样本中的 p53 表达没有差异，这表明 p53 表达增加不是 LS 病变进展为 SCC 的决定因素。
