Ottawa Institute of Systems Biology, Department of Biochemistry, University of Ottawa, Ottawa, Ontario, Canada.
Nat Struct Mol Biol. 2011 Jun 5;18(7):857-9. doi: 10.1038/nsmb.2093.
Absent, small or homeotic discs-like 2 (ASH2L) is a trithorax group (TrxG) protein and a regulatory subunit of the SET1 family of lysine methyltransferases. Here we report that ASH2L binds DNA using a forkhead-like helix-wing-helix (HWH) domain. In vivo, the ASH2L HWH domain is required for binding to the β-globin locus control region, histone H3 Lys4 (H3K4) trimethylation and maximal expression of the β-globin gene (Hbb-1), validating the functional importance of the ASH2L DNA binding domain.
缺失、小或同源异型盘状 2（ASH2L）是一个转录激活因子相关蛋白家族（TrxG）蛋白和 SET1 家族赖氨酸甲基转移酶的调节亚基。在这里，我们报告说 ASH2L 使用叉头样螺旋-环-螺旋（HWH）结构域与 DNA 结合。在体内，ASH2L 的 HWH 结构域对于与β-珠蛋白基因调控区、组蛋白 H3 赖氨酸 4（H3K4）三甲基化和β-珠蛋白基因（Hbb-1）的最大表达的结合是必需的，验证了 ASH2L DNA 结合域的功能重要性。
