Department of Electrical and Biomedical Engineering, University of Nevada Reno, Reno, Nevada 89557-0260, USA.
Analyst. 2011 Jul 21;136(14):2975-80. doi: 10.1039/c1an15274b. Epub 2011 Jun 6.
In this work, a fluorescence signal transduction mechanism based on cation release from ZnS nanocrystals was developed for sandwich immunoassay. In this mechanism, ZnS nanocrystals as labels in immunoassay are dissolved by acid to release zinc ions. After pH adjustment of the dissolving solution using a basic solution, zinc-ion sensitive fluorescence indicator Fluozin-3 is added to bind with the released zinc ions for sensitive fluorescence measurement. Using mouse IgG as a model analyte, the immunoassay adopting this signal transduction mechanism demonstrates a low detection limit around 1 pM and a detection range with two orders of magnitude (1 pM to 0.5 nM).
在这项工作中，开发了一种基于 ZnS 纳米晶体中阳离子释放的荧光信号转导机制，用于三明治免疫分析。在该机制中，免疫分析中的 ZnS 纳米晶体作为标记物被酸溶解以释放锌离子。在用碱性溶液调节溶解溶液的 pH 值后，加入锌离子敏感荧光指示剂 Fluozin-3 与释放的锌离子结合，进行灵敏的荧光测量。使用小鼠 IgG 作为模型分析物，采用这种信号转导机制的免疫分析显示出约 1 pM 的低检测限和两个数量级的检测范围（1 pM 至 0.5 nM）。
