Tanizaki E, Horiuchi T, Niikura M, Mikami T, Wakamiya N
Department of Veterinary Pathology, College of Agriculture, University of Osaka Prefecture, Japan.
Avian Dis. 1990 Apr-Jun;34(2):374-80.
In situ hybridization was applied to detect fowlpox virus (FPV) DNA in formalin-fixed paraffin-embedded sections of the skin from infected chickens by using a biotinylated probe and a streptavidin-alkalinephosphatase conjugate. The immunohistochemical examination was applied to compare the distribution of the FPV DNA to that of related antigenic protein in serial sections. In the infected epithelial cells, FPV DNA was detected in cytoplasmic inclusion bodies and in the rest of cytoplasm. Likewise, immunohistochemical examination revealed the virus antigen in cytoplasm. Ultrastructurally, virions were observed in the cytoplasmic inclusion bodies, and immature virus particles were in the rest of the cytoplasm. The study proved restricted distribution of FPV DNA in the cytoplasm.
应用原位杂交技术，通过使用生物素化探针和链霉亲和素 - 碱性磷酸酶复合物，检测感染鸡皮肤经福尔马林固定、石蜡包埋切片中的禽痘病毒（FPV）DNA。应用免疫组织化学检查，在连续切片中比较FPV DNA与相关抗原蛋白的分布情况。在感染的上皮细胞中，在细胞质包涵体和其余细胞质中检测到FPV DNA。同样，免疫组织化学检查显示细胞质中有病毒抗原。在超微结构上，在细胞质包涵体中观察到病毒粒子，在其余细胞质中有未成熟病毒颗粒。该研究证明了FPV DNA在细胞质中的分布受限。
