Ni Qin, Jiang Xia, Jin Ming-juan, Liu Bing, Chen Kun
Department of Epidemiology and Health Statistics, School of Public Health, Zhejiang University, Hangzhou, Zhejiang, 310058 P. R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2011 Jun;28(3):318-22. doi: 10.3760/cma.j.issn.1003-9406.2011.03.018.
To investigate the association between the apoptosis genes CASP3(rs12108497) and CASP9 (rs4646018) polymorphisms and the risk of developing stomach cancer.
In this population-based case-controlstudy, 278 cases with stomach cancer and 278 age (± 5 years), gender, and residential area matched controls were recruited. The genotypes were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The unconditional Logistic regression analysis was utilized to calculate the odds ratios (OR) and 95% confidence intervals (CI).
The individuals with TC, CC genotypes of rs12108497 locus had significantly increased risk of stomach cancer in comparison to those carrying TT genotype (OR= 1.45, 95% CI: 1.01-2.07 for TC; OR= 2.17, 95%CI: 1.15-4.08 for CC). However, the rs4646018 locus of CASP9 gene polymorphism was not related to stomach cancer risk. Compared with the subjects carrying the both low-risk genotypes, those carrying 1 or 2 high-risk genotypes had a noteworthy increased risk of stomach cancer (OR= 1.60, 95% CI: 1.12-2.30). The combined high-risk genotypes appeared to be more evident in subjects of male (OR= 1.62, 95% CI: 1.05-2.49), ever-smokers (OR= 1.87, 95%CI: 1.12-3.12), ever-drinkers (OR= 1.92, 95%CI: 1.02-3.65) and no family history of cancer (OR= 1.78, 95%CI: 1.18-2.68).
The current findings suggest that the polymorphism of CASP3 rs12108497 might be associated with the risk of stomach cancer. However, the CASP9 rs4646018 polymorphism may not be related to the stomach cancer risk.
探讨凋亡基因半胱天冬酶3（CASP3，rs12108497）和半胱天冬酶9（CASP9，rs4646018）多态性与胃癌发生风险之间的关联。
在这项基于人群的病例对照研究中，招募了278例胃癌患者以及278名年龄（±5岁）、性别和居住地区相匹配的对照。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法分析基因型。运用非条件Logistic回归分析计算比值比（OR）和95%置信区间（CI）。
与携带TT基因型的个体相比，rs12108497位点具有TC、CC基因型的个体患胃癌的风险显著增加（TC基因型：OR = 1.45，95%CI：1.01 - 2.07；CC基因型：OR = 2.17，95%CI：1.15 - 4.08）。然而，CASP9基因多态性的rs4646018位点与胃癌风险无关。与携带两种低风险基因型的受试者相比，携带1种或2种高风险基因型的受试者患胃癌的风险显著增加（OR = 1.60，95%CI：1.12 - 2.30）。联合高风险基因型在男性受试者（OR = 1.62，95%CI：1.05 - 2.49）、曾经吸烟者（OR = 1.87，95%CI：1.12 - 3.12）、曾经饮酒者（OR = 1.92，95%CI：1.02 - 3.65）以及无癌症家族史的受试者中（OR = 1.78，95%CI：1.18 - 2.68）更为明显。
目前的研究结果表明，CASP3 rs12108497的多态性可能与胃癌风险相关。然而，CASP9 rs4646018多态性可能与胃癌风险无关。
