Fagioli M, Ciccone E, Bottino C, Falini B, Grignani F, Moretta A, Moretta L, Pelicci P G
Istituto di Clinica Medica I, University of Perugia, Policlinico Monteluce, Italy.
Blood. 1990 Jul 15;76(2):279-84.
We identified two types of gamma/delta T-cell clones: (1) BB3+ clones, which express the disulphide-linked form of the gamma/delta heterodimer (type 1 receptor) and carry rearrangements of the C gamma 1 gene; and (2) delta TCS1+ clones, which express the nondisulphide-linked form of the gamma/delta heterodimer (type 2 receptor) and carry rearrangements of the C gamma 2 gene. To determine the joining (J) and variable (V) region usage of the two receptor types, T gamma and T delta gene rearrangements and expression were analyzed in each gamma/delta T-cell clone. The results showed that the J and V regions used by the two receptor types are distinct: clones that express the type 1 receptor carry potentially functional rearrangements in J gamma P-V gamma 9 for gamma and in J delta 1-V delta 2 for delta; and clones that express the type 2 receptor had potentially functional rearrangements in J gamma 2-V gamma 2/V gamma 4 for gamma and in J delta 1-V delta 1 for delta. Therefore, two clearly different gamma/delta receptors (V gamma 9-J gamma P-C gamma 1/V delta 2-J delta 1-C delta and V gamma 2/V gamma 4-J gamma 2-C gamma 2/V delta 1-J delta 1-C delta) predominate in peripheral blood.
我们鉴定出两种γ/δ T细胞克隆类型：（1）BB3+克隆，其表达γ/δ异二聚体的二硫键连接形式（1型受体）并携带Cγ1基因重排；（2）δTCS1+克隆，其表达γ/δ异二聚体的非二硫键连接形式（2型受体）并携带Cγ2基因重排。为了确定两种受体类型的连接（J）区和可变（V）区的使用情况，对每个γ/δ T细胞克隆中的Tγ和Tδ基因重排及表达进行了分析。结果显示，两种受体类型所使用的J区和V区是不同的：表达1型受体的克隆在γ链的JγP - Vγ9以及δ链的Jδ1 - Vδ2中有潜在功能性重排；而表达2型受体的克隆在γ链的Jγ2 - Vγ2/Vγ4以及δ链的Jδ1 - Vδ1中有潜在功能性重排。因此，两种明显不同的γ/δ受体（Vγ9 - JγP - Cγ1/Vδ2 - Jδ1 - Cδ和Vγ2/Vγ4 - Jγ2 - Cγ2/Vδ1 - Jδ1 - Cδ）在外周血中占主导。
