Schure M R, Murphy R E, Klotz W L, Lau W
Rohm and Haas Company, 727 Norristown Road, Spring House, Pennsylvania, 19477.
Anal Chem. 1998 Dec 1;70(23):4985-95. doi: 10.1021/ac980719d.
Capillary gel electrochromatography is evaluated with an entangled polymer solution which is pumped into the capillary and run under fritless conditions. The polymer used has an acid backbone with grafted hydrophobic segments, the polyacid giving the electroosmotic flow and the hydrophobe segments providing the retentive component. Experimental evaluation of this type of system reveals performance similar to capillary electrophoresis and other forms of electrochromatography. The analysis of plate height data demonstrates that zone broadening is primarily due to diffusion with little contribution from nonequilibrium zone broadening. Hence, operation at high velocities (high voltages) is most desirable as opposed to most chromatographic methods. Some of the advantages of this type of experiment include being able to replace the retentive media in a few minutes, fast and reproducible high-performance separation, and having a retention mechanism similar to reversed-phase liquid chromatography. Disadvantages include a low retentive phase concentration and hence low sample loadability and limited solvent compatibility of the polymer. A number of different separations are demonstrated including separation of alkyl benzoates, alkylphenones, alkylbenzenes, oxidation inhibitors, and PAHs.
采用一种缠结聚合物溶液对毛细管凝胶电色谱进行评估，该溶液被泵入毛细管并在无筛板条件下运行。所使用的聚合物具有带有接枝疏水链段的酸性主链，多元酸产生电渗流，疏水链段提供保留成分。对这种类型系统的实验评估表明其性能与毛细管电泳和其他形式的电色谱相似。塔板高度数据的分析表明，区域展宽主要是由于扩散，非平衡区域展宽的贡献很小。因此，与大多数色谱方法相反，在高速（高电压）下操作是最理想的。这类实验的一些优点包括能够在几分钟内更换保留介质、快速且可重现的高效分离，以及具有与反相液相色谱相似的保留机制。缺点包括保留相浓度低，因此样品负载能力低，以及聚合物的溶剂兼容性有限。展示了多种不同的分离，包括苯甲酸酯、烷基苯酮、烷基苯、抗氧化剂和多环芳烃的分离。
