College of Chemistry, Beijing Normal University, Beijing 100875, China.
Talanta. 2011 Jul 15;85(1):333-8. doi: 10.1016/j.talanta.2011.03.066. Epub 2011 Apr 5.
A new and fast method for haptoglobin phenotyping was developed based on microchip electrophoresis with laser induced fluorescence detection. Haptoglobin phenotypes 1-1 and 2-2 were labeled with fluorescein isothiocyanate. The analyses were performed on glass microchip which was simply treated with sodium dodecyl sulfate. After the optimization of the separation conditions, Hp 1-1 and Hp 2-2 could be differentiated in 150s and the detection limits for Hp 1-1 and Hp 2-2 were 0.39 and 0.62 μg/mL, respectively. Finally, the method was applied to human serum samples from healthy people and liver cancer patients. A decrease in Hp concentration for liver cancer patients was confirmed. Featuring high efficiency, speed, simplicity, the method reveals great potentials for the diagnosis of diseases and proteome research.
基于激光诱导荧光检测的微芯片电泳技术，开发了一种新型快速的触珠蛋白表型分析方法。触珠蛋白表型 1-1 和 2-2 用异硫氰酸荧光素标记。分析在经过简单的十二烷基硫酸钠处理的玻璃微芯片上进行。在优化分离条件后，Hp 1-1 和 Hp 2-2 可在 150s 内得到区分，Hp 1-1 和 Hp 2-2 的检测限分别为 0.39 和 0.62μg/mL。最后，该方法应用于健康人和肝癌患者的人血清样本。证实了肝癌患者的 Hp 浓度降低。该方法具有高效、快速、简单的特点，在疾病诊断和蛋白质组研究方面具有很大的潜力。
